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101.
102.
A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum. 相似文献
103.
营养限制对三种浮游动物的影响 总被引:1,自引:0,他引:1
Nutrient imbalance—a mismatch in nutrient ratios between the available food supply and the demands of consumers—has the potential to be transported up food chains,exposing zooplankton to nutrient limitations.In this study,the response of Calanus sinicus(copepod),Moina mongolica(cladocera),and Brachionus plicatilis(rotifer) to nutrient-limited(no-limited,P-limited,and N-limited) food were evaluated from the perspective of growth,reproduction,and stoichiometric homoeostasis.The results indicated that the growth of three species was suppressed under nutrient-limited(especially P-limited) conditions.However,the effect of nutrient limitations on their reproduction was species-specific.For C.sinicus,the dietary UFA(unsaturated fatty acid) as a major phospholipid component affected their egg production and total FA supporting energy promoted the hatchability of eggs.Furthermore,excess carbon in the diet promoted egg production but reduced hatching success.For M.mongolica,nutritional(P and UFA) and energy(total FA) support affected their fecundity.B.plicatilis fecundity exhibited the same pattern of growth(no-limitedN-limitedP-limited).In terms of stoichiometric homeostasis,B.plicatilis' s elemental compositions were less likely to be affected by nutrient limitations than M.mongolica.We suggest that the effects of nutrient imbalances could potentially become an evolutionary force affecting ecosystem structure and stability in eutrophic waters. 相似文献
104.
陆地水储量的季节性变化是导致地表周期性负荷形变位移的主要因素,有效地剔除地表位移中的陆地水储量影响,是获取地壳构造垂向运动的必要过程.四川地处青藏高原东边缘,地形分区明显,境内以长江水系为主,水资源丰富,研究四川地区地表负荷形变位移,有助于分析陆地水储量的时空分布特性及地壳构造形变信息.本文利用研究区域内59个CORS站的GPS观测数据,计算了CORS站点的垂向位移,并将其与GRACE所得相应结果进行对比分析.结果显示,GPS和GRACE所得垂向位移时间序列的振幅大小整体相符,但存在明显的相位差.GPS站点振幅最大值为12.7 mm,对应HANY站,最小值为1.5 mm,对应SCMX站.GRACE所得的地表垂向位移振幅大小均为3~4 mm,且最大位移集中出现在7-9月份;而GPS站点出现最大位移的月份和地形相关,东部盆地、西北部高原和南部山地分别出现在7-8月份、10-11月份和10月份.GPS站点时间序列中的周年项与陆地水的季节性变化强相关,为了讨论陆地水储量对GPS站点位移的影响,本文利用改进的总体经验模态分解方法(MEEMD:Modified Ensemble Empirical Mode Decomposition),从GPS垂向位移时间序列中提取出周年项及约2年的年际变化项.发现利用MEEMD获取的周年项改正原始GPS时间序列时可使其WRMS(Weight Root Mean Square)减少量减小约26%,结果优于最小二乘拟合方法提取的GPS周年项改正效果,验证了MEEMD方法在GPS坐标时间序列处理中的可行性及有效性. 相似文献
105.
将土体视为固-液两相介质,基于饱和土体有效应力原理,建立饱和土体-地下综合管廊结构体系相互作用动力模型:在地应力平衡的静力状态下采用Duncan-Chang非线性弹性本构模型,在地震波作用的动力状态下采用Davidenkov非线性黏弹性本构模型;考虑饱和土体黏弹性动力人工边界条件,将地震动作用转化为作用在人工边界节点上的动力荷载。模型考察不同地震波时程、地震波加速度峰值、入射角度、孔隙率以及地应力场的影响,得出如下结论:(1)地震波的卓越周期与场地卓越周期相近时引起结构上的变形最大;随着地震波加速度峰值的增大结构变形增大;随着地震波入射角度的增加结构变形增大,地震波斜入射情况下产生的行波效应使得结构变形最大。(2)土体材料的孔隙水压力是影响地震中结构变形的主要因素之一。(3)将土体材料考虑为单相介质时结构上的变形要比考虑为固-液两相介质时大得多,直接将饱和土体场地中得到的地震波等效荷载施加到单相土介质-结构动力相互作用模型上,能够得到与完全基于有效应力法一致的结果。 相似文献
106.
为确定容果地区二长花岗岩的形成时代、成因及其地质意义,对容果地区二长花岗岩进行了岩相学、LA-ICP-MS锆石U-Pb年龄及地球化学研究。结果表明: 二长花岗岩中的锆石为岩浆成因,206Pb/238U年龄加权平均值为(69±1) Ma,侵入时代属于晚白垩世; 二长花岗岩中Al2O3含量为13.83%~14.41%,Na2O含量为3.68%~3.94%,K2O含量为4.28%~4.35%, K2O/Na2O为1.08~1.18,相对富钾,铝饱和指数A/CNK平均为1.02,属弱过铝质岩石; 微量元素特征表现为相对富集Rb、U、K等大离子亲石元素,亏损Nb、Ta、Ti等高场强元素的特征。结合区域构造演化背景,可得出在80~69 Ma之间,该区的岩浆活动并未停止,仍然持续在发生,所生成的岩体是新特提斯洋壳俯冲的岩浆产物,形成于造山环境下,形成过程贯穿整个造山运动。 相似文献
107.
区域中长期地震危险性数值分析研究,需要对其初始构造应力场有所了解,但目前以及未来一段时期内仍无法直接观测到深部孕震层区域的应力场状况.本文首先基于岩石库仑-摩尔破裂准则,利用青藏高原及邻区百年历史范围内的强震信息,来反演估算该区域的初始应力场.然后,考虑区域构造应力加载及强震造成的应力扰动共同作用,重现了历史强震的发展过程.然而对于初始应力场的反演估算,本文仅能给出区域其上下限的极限值,并不能唯一确定.因此,采用Monte Carlo随机法,进行大量独立的随机试验计算,生成数千种有差异的区域初始应力场模型,且保证每种模型都能令历史强震有序发生,但未来应力场演化过程不尽相同.最后,将数千种模型在未来时间段内的危险性预测结果集成为数理统计结果,据此给出了区域未来的地震危险性概率分布图.初步结果显示未来强震危险性概率较高地区集中在巴颜喀拉块体边界及鲜水河断裂带地区. 相似文献
108.
目前,在远海开阔性海域磁测中,尚难以有效设立日变站,致使日变改正仍存在着困难.本文利用地磁台站实测资料对地磁日变的纬度分布特征进行研究,发现日变值随纬度变化具有由Sq电流中心向南北两侧递增的规律,且二者之间的关系可用二次函数近似描述。以此规律为基础我们建立了纬度改正方法,该方法以经度链为基站,利用基站数据得到日变值与磁纬度的拟合函数以进行纬度改正,再调整时差作为经度校正即得到计算站的日变值.实测数据计算结果表明,相较于加权平均法,此方法在远距离(经度差达40°)仍能保持较高的校正精度(4 nT),因而能更好地适用于远海磁测. 相似文献
109.
Huan Mi Cédric G. Fichot Karin R. Bryan Gang Qiao Sergio Fagherazzi 《地球表面变化过程与地形》2020,45(15):3780-3790
Rapid water level rise due to climate change has the potential to remobilize loose sediments along shorelines and increase the turbidity of nearshore waters, thereby impacting water quality and aquatic ecosystem health. Siling Lake is one of the largest and most rapidly expanding lakes on the Tibetan Plateau. Between 2000 and 2017, this lake experienced an increase in water level of about 8 m and a doubling in water turbidity. Here, using this lake as a study site, we used a wave model and high-resolution remote sensing of turbidity (Landsat-8) to assess the potential connection between water-level rise, enhanced wind-driven sediment resuspension and water turbidity. Our analysis revealed that strong bottom shear stresses triggered by wind-generated waves over newly flooded areas were related to an increase in water turbidity. The spatial variability of Siling Lake turbidity showed a strong dependence on local wind characteristics and fetch. Two factors combined to drive the increase in turbidity: (1) high wave energy leading to high bottom shear stresses, and (2) flooding of unvegetated shallow areas. Using a new relationship between wave energy and turbidity developed here, we expect the increase in turbidity of Siling Lake to taper off in the near future due to the steep landscape surrounding the lake that will prevent further flooding. Our results imply that rising water levels along the coast are not only expected to influence terrestrial ecosystems but could also change water quality. The methodology presented herein could be applied to other shorelines affected by a rapid increase in water level. © 2020 John Wiley & Sons, Ltd. 相似文献
110.
Sandy-muddy transitional beaches (SMT-Beaches), representing the transition from sandy beaches to tidal mudflats, should theoretically develop very different morphological and sedimentological characteristics in river estuaries and in semi-enclosed bays due to their contrasting dynamic sedimentary environments. Evidence, however, is rare in the scientific literature. To reveal these morphological and sedimentary differences, the sand–mud transition (SMT) boundary distribution, beach profiles, and surface and downcore sediment grain-size compositions of 27 SMT-Beaches located along mesotidal to macrotidal coasts of the western Taiwan Strait, southeastern China, were investigated. The results show that typical estuarine SMT-Beaches are mainly characterized by an ambiguous SMT, a long distance between the SMT and the coastline (31–302 m), lower SMT and inflection point altitudes (average –0.76 m and –0.04 m), and lower upper beach gradients (~0.068) with fine sand. Estuarine SMT-Beach sediments display clear interbedded mud and sand layers, implying potential SMT migrations over various timescales. By contrast, typical bay SMT-Beaches are characterized by distinct SMT, a short distance between the SMT and the coastline (11–52 m), higher SMT and inflection point altitudes (~0.24 m and ~0.35 m), and narrower upper beaches with higher gradients (~0.095) and coarse sand. Bay SMT-Beaches present relatively stable sedimentary sequences and a narrow gravel belt surrounding the inflection point and/or SMT. These morphological and sedimentary differences between the two SMT-Beach types are initially constrained by sediment supply and transport and are further affected by tide conditions and wave climate. Sediment supply and transport predominately control the sediment structures, while the tidal range strongly influences spatial variations in SMT distances. Wave climate normally drives SMT altitude variations. This study highlights the morphological and sedimentary differences in SMT-Beaches in estuaries and bays, providing important knowledge for further revealing their morphodynamic processes and potential future nourishment. © 2020 John Wiley & Sons, Ltd. 相似文献